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  Indian J Med Microbiol
 

Figure 2: (a) Morphological changes in MDA-MB-231 cells after tellurite treatment using Nikon eclipsed phase contrast microscope, and all three independent experiments were performed under identical condition. Morphology of cells sharply (remarkably) changed with increasing conc. of Te and time. Treated cells changed their morphology at the start all cells were rounded and detached later mostly cells are attached and changed their shape from spherical to spindle, invasive, the size of cell increases, flattened, vacuolization of cytoplasm, cells become more elongated and healthy cell becomes more flattened and highly spreader shape with more prominent and visible with increasing volume of cell attached to surface in low concentration as compared to control and their no reduced with increasing concentration of Te; as concentration increases cells goes to rounded small shaped and detached as dying cells, fewer number of cells changed their shape and mostly remain in round later formed debris at high concentrations of Te; and death of cells by apoptosis and necrotic process inducing cell shrinkage, membrane integrity loosed, condensed chromatin, condensed cytoplasm, fragmented nucleus material and in large number of the cells detached from surface and formed apoptotic bodies. (b) Effect of tellurite concentration on MDA-MB-231 cells survival

Figure 2: (a) Morphological changes in MDA-MB-231 cells after tellurite treatment using Nikon eclipsed phase contrast microscope, and all three independent experiments were performed under identical condition. Morphology of cells sharply (remarkably) changed with increasing conc. of Te and time. Treated cells changed their morphology at the start all cells were rounded and detached later mostly cells are attached and changed their shape from spherical to spindle, invasive, the size of cell increases, flattened, vacuolization of cytoplasm, cells become more elongated and healthy cell becomes more flattened and highly spreader shape with more prominent and visible with increasing volume of cell attached to surface in low concentration as compared to control and their no reduced with increasing concentration of Te; as concentration increases cells goes to rounded small shaped and detached as dying cells, fewer number of cells changed their shape and mostly remain in round later formed debris at high concentrations of Te; and death of cells by apoptosis and necrotic process inducing cell shrinkage, membrane integrity loosed, condensed chromatin, condensed cytoplasm, fragmented nucleus material and in large number of the cells detached from surface and formed apoptotic bodies. (b) Effect of tellurite concentration on MDA-MB-231 cells survival